Journal: Molecular Neurobiology

Article Title: Unraveling Cannabidiol's Dual Modulatory Role in Schizophrenia: Network Pharmacology and In Vivo Validation of Neuroinflammatory and Behavioral Modulation

doi: 10.1007/s12035-025-05608-8

Figure Lengend Snippet: CBD intervention reverses LPS-induced downregulation of 5HT1AR and associated proteins expression. ( A ) Representative Western blot analysis showing protein levels of 5-HT1AR, ERK1/2, phosphorylated ERK1/2 (P-ERK1/2), p38, phosphorylated p38 (P-p38), and Ap-1, with GAPDH as a loading control, in cells treated with LPS and/or CBD. ( B ) Quantification of 5-HT1AR expression normalized to GAPDH. ( C , D ) Relative expression of phosphorylated ERK1 ( C ) and ERK2 ( D ) normalized to total ERK1/2 protein. ( E ) Quantification of phosphorylated p38 relative to total p38. ( F ) Relative expression of Ap-1 protein normalized to GAPDH. Data were analyzed by one-way ANOVA, followed by Tukey’s multiple comparisons test. *, Compared to the control group; #, compared to LPS treated group. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, ## p < 0.01, ### p < 0.001, and #### p < 0.0001

Article Snippet: The size-cut membrane was blocked in TBST with 5% non-fat milk for 2 h at room temperature and then incubated with primary antibodies including: rabbit anti-5HT1A Receptor (1:1000 dilution; cat. no. ab85615, Abcam, MA, USA/cat. no. GTX104703, GeneTex, CA, USA), anti-p38 MAPK(1:1000 dilution; cat. no. 8960, Cell Signaling Technology, MA, USA), Phospho-p38 MAPK (Thr180/Tyr182) (1:1000 dilution; cat. no. 8960, Cell Signaling Technology, MA, USA), anti-ERK1/2(1:1000 dilution; cat. no. 4695 T, Cell Signaling Technology, MA, USA), Phospho-ERK1/2(Thr202/Tyr204) (1:1500 dilution; cat. no. 4370 T, Cell Signaling Technology, MA, USA), anti-AP-1(1:1000 dilution; cat. no. 9165 T, Cell Signaling Technology, MA, USA), GAPDH(1:2500 dilution; cat. no. ab9485, Abcam, MA, USA) and β-actin (1:20,000 dilution, cat. no. T0022, Affinity, OH, USA), overnight at 4 °C.

Techniques: Expressing, Western Blot, Control

Journal: Cancer Medicine

Article Title: Elimination of radiation‐induced senescent cancer cells and stromal cells in vitro by near‐infrared photoimmunotherapy

doi: 10.1002/cam4.7381

Figure Lengend Snippet: Cellular senescence induced by γ ‐ray irradiation of A549 and MIA PaCa‐2 cells. (A) The expression profiles of cellular senescence‐related proteins and EGFR were analyzed by immunoblotting. Actin was used as a loading control. Numbers beneath each lane represent densitometric ratios of each protein normalized to that of Day 0. (B) Representative differential interference contrast (DIC) and fluorescence images of A549 and MIA PaCa‐2 cells after irradiation at 10 Gy. SPiDER‐βGal was used for the detection of senescent cells. Scale bar, 20 μm. (C) Senescence associated β‐galactosidase (SA β‐gal) assay of A549 cells after γ ‐ray irradiation. At least 200 cells were analyzed, and the percentage of SA β‐Gal positive cells was determined. ** p < 0.01 versus 0 Gy (Student's t ‐test). TK1, thymidine kinase 1.

Article Snippet: Anti–thymidine kinase 1 (TK1) and anti‐beta‐actin antibodies were purchased from Proteintech (Rosemont, IL, USA).

Techniques: Irradiation, Expressing, Western Blot, Control, Fluorescence